ABSTRACT
The comprehensive university has the advantage of wide range of disciplines as well as rich teaching resources.Integrating and sharing teaching resources among similar disciplines is the direction of development in higher education.Tongji university was taken as an example.Medical college and life sciences college of Tongji university have tried to construct and share the laboratory teaching resource platform.The construction experiences and the effects were summarized.The methods and strategies used in integrating teaching resources of the two colleges were discussed from the perspective of laboratory construction,which provided references for building teaching resource platform in comprehensive university.
ABSTRACT
AIM To study the effect of NO on dependence and tolerance of NM in the guinea pig ileum in vitro. METHODS The segment of the guinea pig ileum were suspended in organ chambers for measuring withdrawal responses are expressed as ratio of naloxone(100 nmol·L-1)and Ach (100 nmol*L-1) contraction respenses and measuring inhibitory effects of NM for contractions evoked by electric stimulation. RESULTS After the segment of the ileum was incubated with NM (200 nmol*L-1), NM (200 nmol·L-1) plus L-Arg (0.1 mmol·L-1) ,at 4℃ for 24 h respectively, the ratio are 0.89±0.02, 0.90±0.02, (n=6) P>0.05. The ratio of NM (200 nmol·L-1) plus NOArg (0.1 mmol·L-1) is 0.47±0.05,compared with control (n=6,P<0.01). After the segment of the ileum was incubated with NM (500 nmol·L-1), NM (500 nmol·L-1) plus L-Arg (0.1 mmol·L-1) at 37℃ for 3 h respectively,the sensitivities of the preparations for NM were reduced significantly,their concentration-response curves were shift to right (P<0.01).In the segment of the ileum incubaled with NM (500 nmol·L-1) and NG-nitro-L-Arginime ( NOArg 0.1 mmol·L-1) at 37℃ for 3 h,the sensitivities of the preparations for NM was unchange (P>0.05).CONCLUSIONS These results suggest that NO has important role on dependence and tolerance of NM in the guinea pig ileum.
ABSTRACT
0.05. The ratio of NM (200 nmol? L-l) plus NOArg (0.1 mmol. L-1 ) is 0.47 ? 0.05, compared with control (n = 6, P 0 .05). CONCLUSIONS These results suggest that NO has important role on dependence and tolerance of NM in the guinea Pig ileum.